Welcome to the Course page for Enzymology HBC 203
Students will obtain basic knowledge about the relationship between properties and structure of the enzymes, their mechanism of action and kinetics of enzymatic reactions. They should be able to characterize the enzymes in each enzymatic class, examples of such enzymes and their application in practice. They should understand the regulatory mechanisms of enzyme activity, enzyme activators and inhibitors. In the second part of the lectures will aquire. They have knowledge in the field of biosensors and immobilized systems. At the end of the course will be presented use of enzymes in medicine, food, organic synthesis, genetics and other areas sectors.
Course Textbook
Biochemistry, 2nd Edition. 2003. The Chemical Reactions of Living Cells.ISBN: 9780080924717.
Christopher K. Mathews, Kensal E. van Holde, Dean R Appling, Spencer J. Anthony-Cahill, Pearson. Biochemistry, 4th Edition. 2013. ISBN-13: 9780132775045
Donald Voet, Judith G. Voet , Charlotte W. Pratt. Fundamentals of Biochemistry: Life at the Molecular Level 5th Edition. 2005. ISBN-13 : 978-0470547847
Jeremy M. Berg, Lubert Stryer, John Tymoczko, Gregory Gatto. 2019, Biochemistry (9th Edition). WH Freeman. ASIN : B07MF2XWSF
Lehninger Principles of Biochemistry 7th Edition. 2017, by David L. Nelson. ISBN-13 : 978-1464126116
Course Description
1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Pre-requisite
CODE: Mathematics, Organic chemistry, Chemical kinetics
Students will obtain basic knowledge about the relationship between properties and structure of the enzymes, their mechanism of action and kinetics of enzymatic reactions. They should be able to characterize the enzymes in each enzymatic class, examples of such enzymes and their application in practice. They should understand the regulatory mechanisms of enzyme activity, enzyme activators and inhibitors. In the second part of the lectures will aquire. They have knowledge in the field of biosensors and immobilized systems. At the end of the course will be presented use of enzymes in medicine, food, organic synthesis, genetics and other areas sectors.
Course Textbook
Biochemistry, 2nd Edition. 2003. The Chemical Reactions of Living Cells.ISBN: 9780080924717.
Christopher K. Mathews, Kensal E. van Holde, Dean R Appling, Spencer J. Anthony-Cahill, Pearson. Biochemistry, 4th Edition. 2013. ISBN-13: 9780132775045
Donald Voet, Judith G. Voet , Charlotte W. Pratt. Fundamentals of Biochemistry: Life at the Molecular Level 5th Edition. 2005. ISBN-13 : 978-0470547847
Jeremy M. Berg, Lubert Stryer, John Tymoczko, Gregory Gatto. 2019, Biochemistry (9th Edition). WH Freeman. ASIN : B07MF2XWSF
Lehninger Principles of Biochemistry 7th Edition. 2017, by David L. Nelson. ISBN-13 : 978-1464126116
Course Description
1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
Pre-requisite
CODE: Mathematics, Organic chemistry, Chemical kinetics
- Teacher: DR. GERALD JUMA JUMA
- Teacher: PROF. FRANCIS JAKIM MULAA MULAA